Stefan Rödiger
@pangolin  ·  activity timestamp 2 days ago

⁂ Article

DGKM in Landshut

During the past few days I was at the DGKMH conference in Landshut – what a nice event! 🎉

2025 | 12. - 13. September
43. Jahrestagung der Deutschen Gesellschaft für klinische Mikrozirkulation und Hämorheologie e.V.

Next one is in Passau (Germany).

With such an interdisciplinary audience, every session feels especially engaging. It is a small conference which is also very supportive for young researachers.

We presented our work: **“Coupling Chemistry Matters: Hybridization and Melting Differences of Oligonucleotides on Microbeads”** (Hafiz Muhammad Husnain Azam, Peter Schierack, Stefan Rödiger). In this study we show how the chemistry used to attach short single‑stranded oligonucleotides (e.g., miRNA) to microbeads influences hybridization efficiency and melting behavior. This is crucial for anyone working with microfluidics, bead‑based assays, microarrays, or related bioanalytical/biosensing techniques.

Our goal: achieve the most sensitive and specific **amplification‑free** detection of miRNAs under isothermal conditions. Our data reveal that covalent coupling via EDC strongly perturbs the nucleic acid, making sensitive detection at 37–45 °C unnecessarily difficult. EDC is very commonly used because it is a simple, robust and costefficent method to In contrast, RNA:DNA hybrids in solution or those coupled with biotin‑streptavidin perform markedly better.

We’re currently finalizing our manuscript and look forward to sharing more details soon! 📚✨


#DGKMH #Landshut #Microbeads #miRNA #Hybridization #MeltingCurves #Bioanalytics #Biosensing #Microfluidics #IsothermalDetection #NucleicAcids #Research #ScienceCommunication #OpenScience 🚀

This research investigates different methods – covalent and non-covalent (streptavidin-biotin) coupling – for attaching capture probes to microbeads used in miRNA detection assays. The key finding is that the non-covalent method yields smoother melting curves and significantly higher hybridization efficiency (90% vs 60% for covalent binding). This seemingly technical detail has important implications for developing robust and reliable bioanalytical tests, particularly those targeting microRNAs as biomarkers for neurodegenerative diseases or other conditions. The variability observed in the covalent coupling method, likely due to secondary amines on the oligonucleotides affecting melting behavior, introduces potential inconsistencies in test results. Smoother melting curves achieved with non-covalent coupling suggest more consistent and predictable binding of target miRNAs to the microbeads. This translates directly into improved analytical sensitivity – meaning the ability to detect even low concentrations of miRNA biomarkers – and greater accuracy in quantification. For bioanalytical tests intended for clinical diagnostics or biomedical research, these improvements are crucial. Reliable detection of subtle changes in miRNA levels can be vital for early disease diagnosis, monitoring treatment response, or understanding disease progression. By optimizing the immobilization strategy on microbeads to ensure efficient and consistent hybridization, this study contributes to developing more dependable and sensitive bioanalytical tools that can ultimately advance our ability to diagnose and manage a wide range of diseases.
This research investigates different methods – covalent and non-covalent (streptavidin-biotin) coupling – for attaching capture probes to microbeads used in miRNA detection assays. The key finding is that the non-covalent method yields smoother melting curves and significantly higher hybridization efficiency (90% vs 60% for covalent binding). This seemingly technical detail has important implications for developing robust and reliable bioanalytical tests, particularly those targeting microRNAs as biomarkers for neurodegenerative diseases or other conditions. The variability observed in the covalent coupling method, likely due to secondary amines on the oligonucleotides affecting melting behavior, introduces potential inconsistencies in test results. Smoother melting curves achieved with non-covalent coupling suggest more consistent and predictable binding of target miRNAs to the microbeads. This translates directly into improved analytical sensitivity – meaning the ability to detect even low concentrations of miRNA biomarkers – and greater accuracy in quantification. For bioanalytical tests intended for clinical diagnostics or biomedical research, these improvements are crucial. Reliable detection of subtle changes in miRNA levels can be vital for early disease diagnosis, monitoring treatment response, or understanding disease progression. By optimizing the immobilization strategy on microbeads to ensure efficient and consistent hybridization, this study contributes to developing more dependable and sensitive bioanalytical tools that can ultimately advance our ability to diagnose and manage a wide range of diseases.
This research investigates different methods – covalent and non-covalent (streptavidin-biotin) coupling – for attaching capture probes to microbeads used in miRNA detection assays. The key finding is that the non-covalent method yields smoother melting curves and significantly higher hybridization efficiency (90% vs 60% for covalent binding). This seemingly technical detail has important implications for developing robust and reliable bioanalytical tests, particularly those targeting microRNAs as biomarkers for neurodegenerative diseases or other conditions. The variability observed in the covalent coupling method, likely due to secondary amines on the oligonucleotides affecting melting behavior, introduces potential inconsistencies in test results. Smoother melting curves achieved with non-covalent coupling suggest more consistent and predictable binding of target miRNAs to the microbeads. This translates directly into improved analytical sensitivity – meaning the ability to detect even low concentrations of miRNA biomarkers – and greater accuracy in quantification. For bioanalytical tests intended for clinical diagnostics or biomedical research, these improvements are crucial. Reliable detection of subtle changes in miRNA levels can be vital for early disease diagnosis, monitoring treatment response, or understanding disease progression. By optimizing the immobilization strategy on microbeads to ensure efficient and consistent hybridization, this study contributes to developing more dependable and sensitive bioanalytical tools that can ultimately advance our ability to diagnose and manage a wide range of diseases.
Stefan Rödiger
@pangolin  ·  activity timestamp 2 days ago

⁂ Article

DGKM in Landshut

During the past few days I was at the DGKMH conference in Landshut – what a nice event! 🎉

2025 | 12. - 13. September
43. Jahrestagung der Deutschen Gesellschaft für klinische Mikrozirkulation und Hämorheologie e.V.

Next one is in Passau (Germany).

With such an interdisciplinary audience, every session feels especially engaging. It is a small conference which is also very supportive for young researachers.

We presented our work: **“Coupling Chemistry Matters: Hybridization and Melting Differences of Oligonucleotides on Microbeads”** (Hafiz Muhammad Husnain Azam, Peter Schierack, Stefan Rödiger). In this study we show how the chemistry used to attach short single‑stranded oligonucleotides (e.g., miRNA) to microbeads influences hybridization efficiency and melting behavior. This is crucial for anyone working with microfluidics, bead‑based assays, microarrays, or related bioanalytical/biosensing techniques.

Our goal: achieve the most sensitive and specific **amplification‑free** detection of miRNAs under isothermal conditions. Our data reveal that covalent coupling via EDC strongly perturbs the nucleic acid, making sensitive detection at 37–45 °C unnecessarily difficult. EDC is very commonly used because it is a simple, robust and costefficent method to In contrast, RNA:DNA hybrids in solution or those coupled with biotin‑streptavidin perform markedly better.

We’re currently finalizing our manuscript and look forward to sharing more details soon! 📚✨


#DGKMH #Landshut #Microbeads #miRNA #Hybridization #MeltingCurves #Bioanalytics #Biosensing #Microfluidics #IsothermalDetection #NucleicAcids #Research #ScienceCommunication #OpenScience 🚀

This research investigates different methods – covalent and non-covalent (streptavidin-biotin) coupling – for attaching capture probes to microbeads used in miRNA detection assays. The key finding is that the non-covalent method yields smoother melting curves and significantly higher hybridization efficiency (90% vs 60% for covalent binding). This seemingly technical detail has important implications for developing robust and reliable bioanalytical tests, particularly those targeting microRNAs as biomarkers for neurodegenerative diseases or other conditions. The variability observed in the covalent coupling method, likely due to secondary amines on the oligonucleotides affecting melting behavior, introduces potential inconsistencies in test results. Smoother melting curves achieved with non-covalent coupling suggest more consistent and predictable binding of target miRNAs to the microbeads. This translates directly into improved analytical sensitivity – meaning the ability to detect even low concentrations of miRNA biomarkers – and greater accuracy in quantification. For bioanalytical tests intended for clinical diagnostics or biomedical research, these improvements are crucial. Reliable detection of subtle changes in miRNA levels can be vital for early disease diagnosis, monitoring treatment response, or understanding disease progression. By optimizing the immobilization strategy on microbeads to ensure efficient and consistent hybridization, this study contributes to developing more dependable and sensitive bioanalytical tools that can ultimately advance our ability to diagnose and manage a wide range of diseases.
This research investigates different methods – covalent and non-covalent (streptavidin-biotin) coupling – for attaching capture probes to microbeads used in miRNA detection assays. The key finding is that the non-covalent method yields smoother melting curves and significantly higher hybridization efficiency (90% vs 60% for covalent binding). This seemingly technical detail has important implications for developing robust and reliable bioanalytical tests, particularly those targeting microRNAs as biomarkers for neurodegenerative diseases or other conditions. The variability observed in the covalent coupling method, likely due to secondary amines on the oligonucleotides affecting melting behavior, introduces potential inconsistencies in test results. Smoother melting curves achieved with non-covalent coupling suggest more consistent and predictable binding of target miRNAs to the microbeads. This translates directly into improved analytical sensitivity – meaning the ability to detect even low concentrations of miRNA biomarkers – and greater accuracy in quantification. For bioanalytical tests intended for clinical diagnostics or biomedical research, these improvements are crucial. Reliable detection of subtle changes in miRNA levels can be vital for early disease diagnosis, monitoring treatment response, or understanding disease progression. By optimizing the immobilization strategy on microbeads to ensure efficient and consistent hybridization, this study contributes to developing more dependable and sensitive bioanalytical tools that can ultimately advance our ability to diagnose and manage a wide range of diseases.
This research investigates different methods – covalent and non-covalent (streptavidin-biotin) coupling – for attaching capture probes to microbeads used in miRNA detection assays. The key finding is that the non-covalent method yields smoother melting curves and significantly higher hybridization efficiency (90% vs 60% for covalent binding). This seemingly technical detail has important implications for developing robust and reliable bioanalytical tests, particularly those targeting microRNAs as biomarkers for neurodegenerative diseases or other conditions. The variability observed in the covalent coupling method, likely due to secondary amines on the oligonucleotides affecting melting behavior, introduces potential inconsistencies in test results. Smoother melting curves achieved with non-covalent coupling suggest more consistent and predictable binding of target miRNAs to the microbeads. This translates directly into improved analytical sensitivity – meaning the ability to detect even low concentrations of miRNA biomarkers – and greater accuracy in quantification. For bioanalytical tests intended for clinical diagnostics or biomedical research, these improvements are crucial. Reliable detection of subtle changes in miRNA levels can be vital for early disease diagnosis, monitoring treatment response, or understanding disease progression. By optimizing the immobilization strategy on microbeads to ensure efficient and consistent hybridization, this study contributes to developing more dependable and sensitive bioanalytical tools that can ultimately advance our ability to diagnose and manage a wide range of diseases.
Astrid Honc-Gruber
@Astrid@fediscience.org  ·  activity timestamp 4 weeks ago

Today marks the conclusion of my internship at @tuberlin 🎓 I had an incredible research experience, thanks to my supervisor, Professor Mario Birkholz (see picture), and my advisors, Professor Anders Henriksson and Phillip Schrenk.

During this time, I learned about the innovative technology of silicon optical sensors 🔬💡 and developed as a researcher by enhancing my data collection methods 📊 and improving the experimental setup. ⚙️ I also honed my skills in interpreting numerical data 📈 and testing hypotheses.

@JLBe
#bioelectronic #microelectronic#biosensor
Stefan Rödiger
@pangolin  ·  activity timestamp 2 days ago

Great that you enjoyed your stay.
Astrid Honc-Gruber
@Astrid@fediscience.org  ·  activity timestamp 4 weeks ago

Today marks the conclusion of my internship at @tuberlin 🎓 I had an incredible research experience, thanks to my supervisor, Professor Mario Birkholz (see picture), and my advisors, Professor Anders Henriksson and Phillip Schrenk.

During this time, I learned about the innovative technology of silicon optical sensors 🔬💡 and developed as a researcher by enhancing my data collection methods 📊 and improving the experimental setup. ⚙️ I also honed my skills in interpreting numerical data 📈 and testing hypotheses.

@JLBe
#bioelectronic #microelectronic#biosensor

opio ⏚
opio ⏚ boosted
Wikipédia en français
@wikipedia_fr@wikis.world  ·  activity timestamp 3 weeks ago

28 août 1963 : Lors de la « Marche sur Washington pour l'emploi et la liberté », le leader du mouvement d'émancipation des noirs américains Martin Luther King prononce son célèbre discours « I have a dream » devant le Lincoln Memorial. #CeJourLà#OnThisDay
https://fr.wikipedia.org/wiki/Marche_sur_Washington_pour_l%27emploi_et_la_libert%C3%A9

Photo de Martin Luther King en train de parler à un podium lors de la marche sur Washington. Plusieurs microphones sont positionnés devant lui. Il y a des visages flous de personnes au premier plan, indiquant une foule ou un public. L’arrière-plan semble être un tissu drapé ou un rideau, il est de couleur claire et uni.
Photo de Martin Luther King en train de parler à un podium lors de la marche sur Washington. Plusieurs microphones sont positionnés devant lui. Il y a des visages flous de personnes au premier plan, indiquant une foule ou un public. L’arrière-plan semble être un tissu drapé ou un rideau, il est de couleur claire et uni.
Photo de Martin Luther King en train de parler à un podium lors de la marche sur Washington. Plusieurs microphones sont positionnés devant lui. Il y a des visages flous de personnes au premier plan, indiquant une foule ou un public. L’arrière-plan semble être un tissu drapé ou un rideau, il est de couleur claire et uni.
Prof. Emily M. Bender(she/her)
@emilymbender@dair-community.social  ·  activity timestamp 3 weeks ago

Mystery AI Hype Theater 3000 Episode 61 “Winning the Race to Hell”

https://www.buzzsprout.com/2126417/episodes/17713221-winning-the-race-to-hell-with-sarah-myers-west-and-kate-brennan-2025-08-04

in which AI Now Institute’s Kate Brennan and Sarah Myers West join me and @alex to wade through the muck that is Trump’s AI Action Plan

Audiogram with Mystery AI Hype Theater 3000 logo, title “Winning the Race to Hell” and subhead “Spoiler alert: Trump’s “AI Action Plan” is a gift to business interests” Subtitles: KATE BRENNAN: We are seeing, first of all, “unleashing innovation” is just how many ways can we talk about how burdensome and hindering regulation is. So, like, immediately, the top line message that we’re getting from this statement is that in order to succeed, in order to win the race, in order to be the best, in order to innovate, we need to take down any sort of regulatory barriers that are hindering us. And then, not only that, we have to use that as justification to pursue this energy dominance, “Build, baby, build,” “Drill, baby, drill” energy agenda. Where linking those two has been, I think, a strategy of the administration since day one. And seeing them, I think, one right after the other makes it so clear that AI is being used rhetorically, strategically, politically, economically, as a wedge to ensure that Big Oil is getting what they want from this just as much as Big Tech is.
Dr. Lucky Tran :verified:
@luckytran@med-mastodon.com  ·  activity timestamp 3 weeks ago

This country’s top scientists & doctors are quitting in droves, signing public letters of no confidence, & were attacked by a conspiracy theorist. It is so dangerous for national security to have our federal health agencies who are tasked with protecting Americans in this state. RFK Jr must resign.
Zack Whittaker
@zackwhittaker@mastodon.social  ·  activity timestamp 3 weeks ago

When reached this morning, a spokesperson for TransUnion refused to answer basic questions about the company's data breach, such as who hosted the third-party application that was breached, how the data was breached (if known), and what types of customers' personal data was stolen.

https://techcrunch.com/2025/08/28/transunion-says-hackers-stole-4-4-million-customers-personal-information/
💙 Obsidian Urbex Photography 💚
@ObsidianUrbex@mstdn.social  ·  activity timestamp 3 weeks ago

Hello, I just moved servers! Here is my little #Introduction. I am Janine, but I go by ObsidianUrbex.

I love exploring A bandoned Places, capturing their unusual beauty both digitally and with #filmphotography

My passion was sparked by a sunset exploration of an abandoned theme park in 2015. I share articles and photos from my adventures on my #website posts (375 so far) and social media.

Looking forward to meeting loads of new #fediverse humans 💙

#Photography#Urbex#Photographer
MikeDunnAuthor
@MikeDunnAuthor@kolektiva.social  ·  activity timestamp 3 weeks ago

Today in Labor History August 28, 1921: The Soviet Red Army dissolved the stateless Anarchist Free Territory, after driving the Black Army out of Ukraine. The anarchist rebel leader, Nester Makhno, barely escaped, and with serious injuries. The Free Territory within Ukraine, also known as Makhnovia (after Nestor Makhno), lasted from 1918 to 1921. It was a stateless, anarchist society that was defended by Makhno’s Revolutionary Insurrectionary Army (AKA the Black Army). Roughly 7 million people lived in the area. The peasants who lived there refused to pay rent to the landowners and seized the estates and livestock of the church, state and private landowners, setting up local committees to manage them and share them among the various villages and communes of the Free State.

#workingclass #LaborHistory #ukraine #anarchism#AnCom#AnarchoCommunism #NestorMakhno #soviet

petersuber
petersuber boosted
Molly White
@molly0xfff@hachyderm.io  ·  activity timestamp 3 weeks ago

I’ve spent the better part of two decades dealing with people trying to dox and harass the volunteers who make Wikipedia the incredible resource it is today.

I liked it better when they weren’t in Congress.

https://oversight.house.gov/wp-content/uploads/2025/08/082725-letter-to-Wikimedia.pdf

#Wikipedia#USpol
LindenShieldARTs
@lindenshieldarts@mastodon.social  ·  activity timestamp 3 weeks ago

Still painting dark forests~

#TraditionalArt#Gouache#Painting#LandscapeArt#Forest#Nature #mosstodon

A photo of an open sketchbook, placed on a wooden desk and surrounded by branches, hag stones, palo santo, and leaves for decoration. The left side of the sketchbook spread shows two gouache paintings, the top (and larger) one featuring a misty conifer forest with mossy ground and bright white back lighting. The bottom one shows pale blue light filtering through a dark conifer forest. The right side also features two gouache paintings; the top one of a very misty, monochrome conifer forest, and the bottom one of a dark conifer forest with mossy ground and pale sunlight coming in from the left.
A photo of an open sketchbook, placed on a wooden desk and surrounded by branches, hag stones, palo santo, and leaves for decoration. The left side of the sketchbook spread shows two gouache paintings, the top (and larger) one featuring a misty conifer forest with mossy ground and bright white back lighting. The bottom one shows pale blue light filtering through a dark conifer forest. The right side also features two gouache paintings; the top one of a very misty, monochrome conifer forest, and the bottom one of a dark conifer forest with mossy ground and pale sunlight coming in from the left.
A photo of an open sketchbook, placed on a wooden desk and surrounded by branches, hag stones, palo santo, and leaves for decoration. The left side of the sketchbook spread shows two gouache paintings, the top (and larger) one featuring a misty conifer forest with mossy ground and bright white back lighting. The bottom one shows pale blue light filtering through a dark conifer forest. The right side also features two gouache paintings; the top one of a very misty, monochrome conifer forest, and the bottom one of a dark conifer forest with mossy ground and pale sunlight coming in from the left.
James Cat
@zz_james@mastodon.social  ·  activity timestamp 3 weeks ago
@pvonhellermannn people often make the mistake of thinking centrism is about being moderate, but under neo-liberalism centrism is a political doctrine in and of itself, it prescribes that a party shall have no ideological basis and merely respond to voter demands as a business responds to customer demands. If you're looking for something progressive, look elsewhere
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